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alexa 488 antirabbit secondary jackson immunoresearch  (Jackson Immuno)


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    Jackson Immuno alexa 488 antirabbit secondary jackson immunoresearch
    Alexa 488 Antirabbit Secondary Jackson Immunoresearch, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 96/100, based on 2636 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/jackson+immunoresearch+secondary+anti+rabbit/Alexa+Fluor+488+AffiniPure+Donkey+Anti-Rabbit+IgG/pm41872551-248-135-138
    Average 96 stars, based on 2636 article reviews
    alexa 488 antirabbit secondary jackson immunoresearch - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Expressing:

    Article Title: Estrogen administration enhances the adverse effects of cigarette smoking on the heart in cycling female mice.
    Article Snippet: Gene expressions were monitored using SYBR® Green PCR Master Mix (Bio-Rad, Hercules, CA, USA). .. GAPDH expression was used to normalize gene expressions between different samples. cDNA was synthesized from RNA using Revert Aid 1st Strand cDNA synthesis kit (Thermo, USA), followed by real-time PCR analysis in a CFX96 real-time PCR instrument (Bio-Rad, Table 1 List of antibodies Antibody (dilution) Catalogue number Commercial source Anti-IL13 (1/500–1/1000) ab106732 abcam Anti-IL4 (1/1000) ab9728 abcam Anti-IL1beta (1/500) ab9722 abcam Anti-IL13 (1/500–1/1000) ab106732 abcam Anti-IL10 (1/1000) ab9969 abcam Anti-PARP-1 (1/500–1/1000) ab227244 abcam Anti-GAPDH (1/500–1/1000) ab8245 abcam Secondary anti-mouse (1:10,000–1:200,000) 115-035-003 Jackson ImmunoResearch Secondary anti-rabbit (1:10,000) ab8245 abcam Germany). ..

    Synthesized:

    Article Title: Estrogen administration enhances the adverse effects of cigarette smoking on the heart in cycling female mice.
    Article Snippet: Gene expressions were monitored using SYBR® Green PCR Master Mix (Bio-Rad, Hercules, CA, USA). .. GAPDH expression was used to normalize gene expressions between different samples. cDNA was synthesized from RNA using Revert Aid 1st Strand cDNA synthesis kit (Thermo, USA), followed by real-time PCR analysis in a CFX96 real-time PCR instrument (Bio-Rad, Table 1 List of antibodies Antibody (dilution) Catalogue number Commercial source Anti-IL13 (1/500–1/1000) ab106732 abcam Anti-IL4 (1/1000) ab9728 abcam Anti-IL1beta (1/500) ab9722 abcam Anti-IL13 (1/500–1/1000) ab106732 abcam Anti-IL10 (1/1000) ab9969 abcam Anti-PARP-1 (1/500–1/1000) ab227244 abcam Anti-GAPDH (1/500–1/1000) ab8245 abcam Secondary anti-mouse (1:10,000–1:200,000) 115-035-003 Jackson ImmunoResearch Secondary anti-rabbit (1:10,000) ab8245 abcam Germany). ..

    cDNA Synthesis:

    Article Title: Estrogen administration enhances the adverse effects of cigarette smoking on the heart in cycling female mice.
    Article Snippet: Gene expressions were monitored using SYBR® Green PCR Master Mix (Bio-Rad, Hercules, CA, USA). .. GAPDH expression was used to normalize gene expressions between different samples. cDNA was synthesized from RNA using Revert Aid 1st Strand cDNA synthesis kit (Thermo, USA), followed by real-time PCR analysis in a CFX96 real-time PCR instrument (Bio-Rad, Table 1 List of antibodies Antibody (dilution) Catalogue number Commercial source Anti-IL13 (1/500–1/1000) ab106732 abcam Anti-IL4 (1/1000) ab9728 abcam Anti-IL1beta (1/500) ab9722 abcam Anti-IL13 (1/500–1/1000) ab106732 abcam Anti-IL10 (1/1000) ab9969 abcam Anti-PARP-1 (1/500–1/1000) ab227244 abcam Anti-GAPDH (1/500–1/1000) ab8245 abcam Secondary anti-mouse (1:10,000–1:200,000) 115-035-003 Jackson ImmunoResearch Secondary anti-rabbit (1:10,000) ab8245 abcam Germany). ..

    Real-time Polymerase Chain Reaction:

    Article Title: Estrogen administration enhances the adverse effects of cigarette smoking on the heart in cycling female mice.
    Article Snippet: Gene expressions were monitored using SYBR® Green PCR Master Mix (Bio-Rad, Hercules, CA, USA). .. GAPDH expression was used to normalize gene expressions between different samples. cDNA was synthesized from RNA using Revert Aid 1st Strand cDNA synthesis kit (Thermo, USA), followed by real-time PCR analysis in a CFX96 real-time PCR instrument (Bio-Rad, Table 1 List of antibodies Antibody (dilution) Catalogue number Commercial source Anti-IL13 (1/500–1/1000) ab106732 abcam Anti-IL4 (1/1000) ab9728 abcam Anti-IL1beta (1/500) ab9722 abcam Anti-IL13 (1/500–1/1000) ab106732 abcam Anti-IL10 (1/1000) ab9969 abcam Anti-PARP-1 (1/500–1/1000) ab227244 abcam Anti-GAPDH (1/500–1/1000) ab8245 abcam Secondary anti-mouse (1:10,000–1:200,000) 115-035-003 Jackson ImmunoResearch Secondary anti-rabbit (1:10,000) ab8245 abcam Germany). ..

    Polymerase Chain Reaction:

    Article Title: Estrogen Administration Enhances the Adverse Effects of Cigarette Smoking on the Heart in Cycling Female Mice
    Article Snippet: Image J software (https://imagej.nih.gov/ij/) was used to quantify the intensity of bands and Page 6/24 to normalize GAPDH protein levels on each group to con rm any up or down-regulation of the targeted protein. .. Table 1 List of antibodies Antibody (dilution) Catalogue number Commercial Source Anti-IL13 (1/500-1/1000) ab106732 abcam Anti-IL4 (1/1000) ab9728 abcam Anti-IL1beta (1/500) ab9722 abcam Anti-IL13 (1/500-1/1000) ab106732 abcam Anti-IL10 (1/1000) ab9969 abcam Anti-PARP-1 (1/500-1/1000) ab227244 abcam Anti-GAPDH (1/500-1/1000) ab8245 abcam Secondary anti-mouse (1:10000–1:200000) 115-035-003 Jackson ImmunoResearch Secondary anti-rabbit (1:10000) ab8245 abcam Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) Snap frozen heart tissues were used for RNA extraction. .. Brie y, mid-sections from the left ventricle were grounded in liquid nitrogen by a mortar and pestle then total RNA was isolated using TRIzol according to manufacturer’s instructions (Thermo Fisher Scienti c, Grand Island, NY, USA).

    RNA Extraction:

    Article Title: Estrogen Administration Enhances the Adverse Effects of Cigarette Smoking on the Heart in Cycling Female Mice
    Article Snippet: Image J software (https://imagej.nih.gov/ij/) was used to quantify the intensity of bands and Page 6/24 to normalize GAPDH protein levels on each group to con rm any up or down-regulation of the targeted protein. .. Table 1 List of antibodies Antibody (dilution) Catalogue number Commercial Source Anti-IL13 (1/500-1/1000) ab106732 abcam Anti-IL4 (1/1000) ab9728 abcam Anti-IL1beta (1/500) ab9722 abcam Anti-IL13 (1/500-1/1000) ab106732 abcam Anti-IL10 (1/1000) ab9969 abcam Anti-PARP-1 (1/500-1/1000) ab227244 abcam Anti-GAPDH (1/500-1/1000) ab8245 abcam Secondary anti-mouse (1:10000–1:200000) 115-035-003 Jackson ImmunoResearch Secondary anti-rabbit (1:10000) ab8245 abcam Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) Snap frozen heart tissues were used for RNA extraction. .. Brie y, mid-sections from the left ventricle were grounded in liquid nitrogen by a mortar and pestle then total RNA was isolated using TRIzol according to manufacturer’s instructions (Thermo Fisher Scienti c, Grand Island, NY, USA).



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